Brain Development

/// Learning in young mice

Litters  are weaned at PND22 and submitted to normal/compound X enriched food until PND60. At PND60 15-18, three different behavioral procedures used:

 

Amylgen---iconeThe learning in the Morris water-maze (assessing spatial learning) from

PND60 to PND64.

Amylgen---iconeThe probe-test (assessing spatial long term memory) at PND65

Amylgen---iconeThe working memory procedure in the Morris water maze (assessing

spatial working memory) from PND66 to PND68.

 

/// Neonatal stress

Pregnant female rats are received in our animal facility at embryonic day 13 (E13).

Amylgen---iconeBetween E17 and E20, they are submitted to prenatal stress. Repeatedly restrained animals are placed in Plexiglas transparent cylinders (20 cm length, 7 cm diameter) under bright light during 90 min per day, for 4 consecutive days. Control dams are also handled but are never placed in restraint tubes.

Amylgen---iconeAt E21/PND1 (post natal day 1), litter is reduced to balanced 10 animals per dam, biological mothers raise their pups.

Amylgen---iconeLitters weaned at PND22 and submitted to normal/compound X enriched food until PND60.

Amylgen---iconeAt PND60 15-18, three different behavioral procedures used:

 

  • The learning in the Morris water-maze (assessing spatial learning) from PND60 to PND64.
  • The probe-test (assessing spatial long term memory) at PND65
  • The working memory procedure in the Morris water maze (assessing

spatial working memory) from PND66 to PND68.