Brain Aging

/// Senescence Accelerated Mice (SAM)

We make available studies on senescence accelerated mice  (SAM), an inbred mouse model, derived from the AKR/J strain that is widely used in studies of aging (Takeda et al., Mechanism of Aging and Development, 1981). The P8 substrain (SAM-P8) of these mice has a markedly shortened life span when compared to the R1 substrain (SAM-R1), which also shows a slower aging process. In parallel with their premature aging, SAM-P8 mice also exhibit increased neurological senescence, immunosenescence, and age-related hematopoietic deficits, which closely mimic typical human aging characteristics. This mouse system, with its homogeneous genetic background, therefore, provides an excellent experimental model for studying aging and antiaging therapeutics.

/// D-galactose (DG)

The injection of D-galactose (DG) into rats or mice is an aging model  we make available to study your products. Chronic treatment with DG for a period of 6 weeks leads to the acceleration of senescence and a shortened lifespan. Animals show cognitive dysfunction, neurologic impairment associated with the increases of brain oxidative stress and the decreases of antioxidant enzyme activity, cholinergic degeneration, impairment of synaptic plasticity and neurogenesis, altered expression of amyloid-beta metabolism-associated molecules, reactive gliosis and neuroinflammation.

/// Aged animals

Upon request of our customers we may test their products in old animals 18-24 old mice or rats.

Following two months of compound or vehicle administration, cognitive ability of the test product is assessed blind to treatment group in the MWM.

Following behavioral testing, animals are sacrificed for immunohistochemical detection of BrdU in order to assess the effect on neurogenesis.

The effect of the treatment is also evaluated on the evolution of body weight which is known to decline steadily with age.