Amylgen provides an integrated service : the same animals evaluated for behavior are sampled for plasma, CSF and brain structures that are characterized for various markers using biochemical assays. More precise immunohistochemical analyses may be performed in brain slices.

/// Biomarker analysis following in vivo studies

We offer biomarker analysis of a variety of samples from in vivo studies. Biomarker studies encompass detection of protein or mRNA utilizing ELISA, PCR and IHC.

Sample preparation

Proteins extraction

/// Molecular analyses

Protein contents dosage

…

/// Biochemical analyses

Enzymatic activity

Toxicity assays

Oxidative stress measure by colorimetric determination of lipid peroxidation levels

Specific markers on contractant’s request

/// Histology and Immunohistochemistry

Sample preparation:

– Cryosectioning

– Paraffin sectioning

– Vibratome sectioning

Histological staining:

– Nissl staining

– Cresyl violet

– Congo red

– Fluoro jade B

– …

Immunohistochemical staining:

– Neuronal, astroglial and microglial markers

– Cholinergic markers

– Proliferating cells

– Apoptosis markers

– Specific markers on contractant’s request

– Direct morphological observation

– Fluorescence microscopy

– Confocal microscopy